Liver Enzyme Lab - 25 pts
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by Kathy Egbert
| 25 Questions
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Class Period
A 1st
B 2nd
C 4th
D 5th
E 6th
F 7th
G 8th
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Last Name
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First Name
Background Information

All organisms rely on enzymes to catalyze chemical reactions. An enzyme is a biological catalyst that increases the rate of chemical reactions by lowering the level of activation energy necessary to start the reaction. Without enzymes, many of the chemical reactions that occur within living things would proceed too slowly to be useful. Enzymes speed up these reactions by bringing the reactants into close proximity and facilitating their interaction.

Liver contains a specific enzyme called catalase. When hydrogen peroxide (H202) is added to liver, a chemical reaction happens which results in the production of oxygen gas (O2) and liquid water (H20).

As you watch this Liver Enzyme Lab from school tube, fill out the worksheet and questions.‚Äč We will start and stop the video as needed to complete the worksheet.
Liver Enzyme Lab Worksheet
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q
r
4
1
water and liver
True
False
5
1
6
1
This test tube was the control. A control creates a benchmark or standard to use to compare the other reactions against.
True
False
7
1
A more water
B more liver
C to wait longer
D substrate
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Liver and hydrogen peroxide were put in the second test tube.
True
False
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1
Bubbles formed and rose up inside the test tube.
True
False
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The prediction is that the bubbles have oxygen in them.
True
False
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Since oxygen is flammable, we could see if the bubbles would burn.
True
False
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A only liver
B only water
C only hydrogen peroxide
13
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Bubbles formed and rose to the top of the test tube. A burning wood splint was placed inside the test tube and it caught on fire.
True
False
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A water isn't flammable
B liver isn't flammable
C hydrogen peroxide isn't flammable
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Liver and hydrogen peroxide were added to the 4th test tube.
True
False
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The same reaction as Test Tube #2. When the burning wood splint is put near the bubbles they catch fire. After several times catching fire, the splint burns out.
True
False
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There had to be oxygen in the bubbles for them to burn when the wood splint was placed near them. There is also water in the bubbles because the fire was put out.
True
False
Table continued on the next page...
worksheet continued...
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g
h
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A boiled liver
B water
C hydrogen peroxide
D all three were put in the test tube
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There was no reaction.
True
False
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shape
True
False
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function; meaning it won't work.
True
False
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The temperature wasn't cold enough to denature the enzyme, so there will still be a reaction but it will take longer to react.
True
False
Remember our experiments with dissolving sugar in water. The sugar dissolves faster in hot water than in cold water. The sugar eventually dissolved in the cold water, but it took a lot longer compared to the sugar in the hot water.
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