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Protein Synthesis and Mutations
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by Shoshana Berkovic
| 6 Questions
Note from the author:
students will transcribe a DNA sequence of 30 nitrogen bases to an RNA sequence of codons, use a genetic code chart to translate the codons to an amino acid sequence, then record the effects of a substitution and a deletion mutation on that original DNA sequence.
1
1 pt
Write the sequence of RNA bases that would be transcribed from the sequence of 30 bases below:
TACACGCAATTACCAGGGTAGCCATTGATT
Use the space bar to separate your sequence into codons.
2
1 pt
Use the genetic code chart provided above to translate your sequence of RNA base codons into an amino acid sequence to make a protein. Copy the three letters representing each amino acid as shown on the chart. Separate each of your amino acids from the others with a space.
3
2 pts
Explain what would happen if there was a substitution mutation that changed the 5th base in the DNA from a C to a T. Write the sequence of RNA codons that would result from this kind of mutation. Don't forget to separate each codon with a space.

4
1 pt
Write the sequence of amino acids that would result from this kind of mutation.
5
2 pts
Let's say that there is a deletion mutation that caused that same base in the DNA (the C in the 5th place from the beginning) to be missing from the DNA sequence?
Write the sequence of RNA codons that would result from this kind of mutation.
6
1 pt
Write the sequence of amino acids based on the RNA codons you wrote in #5 that would result from this deletion mutation. (Compare your sequence to the sequence you wrote for #2 - think about how that one missing base affected the protein)
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