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Biological Macromolecules Lab
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by John Lundy
| 9 Questions
Note from the author:
An in depth lab looking at the functional range of the peroxidase (protein) enzyme.
1
1 pt
Take Ten Minutes with your group and research Turnips and the Peroxidase enzyme. Report your findings below.

1pt) general information about turnips is presented

1pt) general information about Peroxidase is presented

2pt) two references are provided as a source for the information.

2
1 pt
Take 5 minutes and write a hypothesis about how temperature will affect the functionality of the peroxidase enzyme. Make sure you clear your hypothesis with Mr. Lundy before proceeding to the next step.

1pt) hypothesis is related to the function of the peroxidase enzyme and temperature

1pt) hypothesis unfalsifiable

1pt) hypothesis was okayed by Mr. Lundy

3
5 pts
Step 1; open an excel file
Step 2: Using the materials provided ad 5ml of hydrogen peroxide to 3 test tube.
Step 3: Measure the temperature of the hydrogen peroxide at room temperature.
Record your temperature in your excel File

Step 4: ready a stop watch then add 1 drop of the peroxidase enzyme to the first testbed and record how long it takes for the reaction to end.
Record your findings in your excel file

Step 5: ready a stop watch then add 1 drop of the peroxidase enzyme to the second testbed and record how long it takes for the reaction to end.
Record your findings in your excel file

Step 6: ready a stop watch then add 1 drop of the peroxidase enzyme to the third testbed and record how long it takes for the reaction to end.
Record your findings in your excel file

Step 7: clean out your test tubes and set them to dry
Step 8: fill the next 3 test tubes with 5ml of hydrogen peroxide.
Step 9: put 10ml of the peroxidase enzyme into the 100 ml beaker
Step 10: place the beaker on the hot plate and turn the heater on low and let sit for 3 minutes
Step 11: after 3 minutes record the temperature of the peroxidase in the beaker and add it to your excel file
Step 12: Repeat steps 4-8 using the peroxidase from the beaker
Record your findings in your excel file

Step 13: Place the beaker of peroxidase back on the hot plate and turn it half way up and let the beaker of peroxidase sit for 3 minutes
Step 14: Repeat steps 4-8 using the peroxidase from the beaker
Record your findings in your excel file

Step 15: place the peroxidase beaker back on the hotplate and turn the hotplate up all the way and let it sit for 3 minutes.
Step 16: Repeat steps 4-8 using the peroxidase from the beaker
Record your findings in your excel file

Step 17: wash out all test tubes and beakers and set to dry.
Step 18:organize your data and then upload an image of or copy and paste your findings below.

1pt) data is from 4 different temperatures

1pt) 3 trials were given per temperature

1pt) data is well organized

1pt) data is well labeld

1pt) data reflects the experament was done correctly
4
2 pts
Calculate the average time for the reaction to finish for each of your trials and create a table with your results below. Be sure to show your calculations.

1pt) calculations shown

1pt) data table shows all 4 averages

Upload an image of or copy and paste your findings below.
5
3 pts
Make 2 graphs, one showing all 12 trials and one showing only the 4 averaged values.

1pt) the correct type of graph is chosen to represent the data

1pt) all 12 trials are correctly graphed on the first graph

1pt) all 4 averages are correctly graphed on the second graph

Upload an image of or copy and paste your findings below.
8
4 pts
Using your reflections, write a formal summer of your findings and how they related to supporting or invalidating your hypothesis.

1pt) data is referenced

1pt) data is explained

1pt) data is related to the hypothesis

1pt) examination of experimental procedure is discussed and areas for improvement are presented

9
1 pt
Based on what you’ve learned, what other factors could be used to test the functional range of the peroxidase enzyme?

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